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SUPER-RESOLUTION ​/ LOCALIZATION-MICROSCOPY

​Single molecule microscopy allows nanometer localization of the detected emitters and subtle probing of their spatio temporal nano-environment including in living cells and tissues.
We are developing several single-molecule based super-resolution strategies (also called "localization microscopy") to achieve fixed and live cell studies of endogenous proteins at ultra-high resolution.
​
In particular, we developed uPAINT microscopy, which is the first implementation of PAINT super-resolution microscopy in fixed and live cells including live neurons.
In addition, uPAINT allowed studying functional receptors in interaction by combining super-resolution microscopy and single-molecule FRET.
​
Current projects aim developing such strategies for studies in thick tissues. 
​

Review articles: 
​

« The 2015 Super-Resolution Microscopy Roadmap »
S. W. Hell, S.J. Sahl, M. Bates, , X. Zhuang, R. Heintzmann, M.J Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S.J. Davis, C. Eggeling, D. Klenerman, K.I. Willig, G. Vicidomini, M. Castello, A. Diaspro, T. Cordes
J. App. Phys. D 48 (2015) 443001

« Super-resolution microscopy approaches for live cell imaging » 
A. Godin, B. Lounis, L. Cognet
Biophys. J., 107 (2014) 1777-1784

« Advances in live-cell single-particle tracking and dynamic super-resolution imaging »
L. Cognet, C. Leduc & B. Lounis
Current Opinion in Chemical Biology 20 (2014) 78-85 

Some achievements: 
Picture




 NEW:

​"Self-interference 3D super-resolution microscopy for deep tissue investigations"
P. Bon, J. Linarès-Loyez, M. Feyeux, K. Alessandri, B. Lounis, P. Nassoy and L. Cognet
Nat. Methods, (2018)
 online
Picture


​« Identification and super-resolution imaging of ligand-activated receptor dimers in live cells » 

P. Winckler, L. Lartigues, G.Gianonne, F. De Giorgi, F. Ichas, J-B. Sibarita, B. Lounis and L. Cognet 
Sci. Rep., 3 (2013) 2387


Picture
 

​« High content Super-Resolution Imaging of Live Cell by uPAINT. »

G. Giannone, E. Hosy, JB. Sibarita, D. Choquet and L. Cognet
Methods Biol Mol 950 (2013) 95-110


Picture

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« Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions. »
O. Rossier, V. Octeau, J.B.  Sibarita, C. Leduc, B. Tessier, D. Nair,  V. Gatterdam, O. Destaing, C. Albigès-Rizo, R. Tampé, L. Cognet, D. Choquet, B. Lounis  & G. Giannone
Nature Cell Biology 14 (2012) 1057-1067


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« Dynamic super-resolution imaging of endogenous proteins on living cells at ultra-high density. »
G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A.I. Sobolevsky, M.P. Rosconi, E. Gouaux, R. Tampé, D. Choquet and L. Cognet
Biophys. J., 99 , 4 (2010) 1303-1310, Featured Article


Picture


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​
 "Sub-diffraction Far-field Imaging of Luminescent Single-Walled Carbon Nanotubes »
L. Cognet, D. Tsyboulski, and R.B. Weisman
Nanoletters, 8, 2 (2008) 749 - 753
​


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  • Home
  • People
  • Publications
  • Research
    • ENSEMBLE PROJECT
    • Super-resolution
    • Single Molecule in Neurosciences
    • SWCNT Optical Microscopy
    • SWCNT Tracking & bio-applications
    • Single Molecule Nanoprobes
    • Photothermal Microscopy
  • About L. COGNET
    • Presentations & videos
  • Fundings
  • Contact us
  • Home
  • People
  • Publications
  • Research
    • ENSEMBLE PROJECT
    • Super-resolution
    • Single Molecule in Neurosciences
    • SWCNT Optical Microscopy
    • SWCNT Tracking & bio-applications
    • Single Molecule Nanoprobes
    • Photothermal Microscopy
  • About L. COGNET
    • Presentations & videos
  • Fundings
  • Contact us