SUPER-RESOLUTION / LOCALIZATION-MICROSCOPY
Single molecule microscopy allows nanometer localization of the detected emitters and subtle probing of their spatio temporal nano-environment including in living cells and tissues.
We are developing several single-molecule based super-resolution strategies (also called "localization microscopy") to achieve fixed and live cell studies of endogenous proteins at ultra-high resolution.
In particular, we developed uPAINT microscopy, which is the first implementation of PAINT super-resolution microscopy in fixed and live cells including live neurons.
In addition, uPAINT allowed studying functional receptors in interaction by combining super-resolution microscopy and single-molecule FRET.
Current projects aim developing such strategies for studies in thick tissues.
Single molecule microscopy allows nanometer localization of the detected emitters and subtle probing of their spatio temporal nano-environment including in living cells and tissues.
We are developing several single-molecule based super-resolution strategies (also called "localization microscopy") to achieve fixed and live cell studies of endogenous proteins at ultra-high resolution.
In particular, we developed uPAINT microscopy, which is the first implementation of PAINT super-resolution microscopy in fixed and live cells including live neurons.
In addition, uPAINT allowed studying functional receptors in interaction by combining super-resolution microscopy and single-molecule FRET.
Current projects aim developing such strategies for studies in thick tissues.
Review articles:
« The 2015 Super-Resolution Microscopy Roadmap »
S. W. Hell, S.J. Sahl, M. Bates, , X. Zhuang, R. Heintzmann, M.J Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S.J. Davis, C. Eggeling, D. Klenerman, K.I. Willig, G. Vicidomini, M. Castello, A. Diaspro, T. Cordes
J. App. Phys. D 48 (2015) 443001
« Super-resolution microscopy approaches for live cell imaging »
A. Godin, B. Lounis, L. Cognet
Biophys. J., 107 (2014) 1777-1784
« Advances in live-cell single-particle tracking and dynamic super-resolution imaging »
L. Cognet, C. Leduc & B. Lounis
Current Opinion in Chemical Biology 20 (2014) 78-85
« The 2015 Super-Resolution Microscopy Roadmap »
S. W. Hell, S.J. Sahl, M. Bates, , X. Zhuang, R. Heintzmann, M.J Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S.J. Davis, C. Eggeling, D. Klenerman, K.I. Willig, G. Vicidomini, M. Castello, A. Diaspro, T. Cordes
J. App. Phys. D 48 (2015) 443001
« Super-resolution microscopy approaches for live cell imaging »
A. Godin, B. Lounis, L. Cognet
Biophys. J., 107 (2014) 1777-1784
« Advances in live-cell single-particle tracking and dynamic super-resolution imaging »
L. Cognet, C. Leduc & B. Lounis
Current Opinion in Chemical Biology 20 (2014) 78-85
Some achievements:
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NEW: "Self-interference 3D super-resolution microscopy for deep tissue investigations" P. Bon, J. Linarès-Loyez, M. Feyeux, K. Alessandri, B. Lounis, P. Nassoy and L. Cognet Nat. Methods, (2018) online |
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« Identification and super-resolution imaging of ligand-activated receptor dimers in live cells » P. Winckler, L. Lartigues, G.Gianonne, F. De Giorgi, F. Ichas, J-B. Sibarita, B. Lounis and L. Cognet Sci. Rep., 3 (2013) 2387 |
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« High content Super-Resolution Imaging of Live Cell by uPAINT. » G. Giannone, E. Hosy, JB. Sibarita, D. Choquet and L. Cognet Methods Biol Mol 950 (2013) 95-110 |
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« Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions. » O. Rossier, V. Octeau, J.B. Sibarita, C. Leduc, B. Tessier, D. Nair, V. Gatterdam, O. Destaing, C. Albigès-Rizo, R. Tampé, L. Cognet, D. Choquet, B. Lounis & G. Giannone Nature Cell Biology 14 (2012) 1057-1067 |
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« Dynamic super-resolution imaging of endogenous proteins on living cells at ultra-high density. » G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A.I. Sobolevsky, M.P. Rosconi, E. Gouaux, R. Tampé, D. Choquet and L. Cognet Biophys. J., 99 , 4 (2010) 1303-1310, Featured Article |
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"Sub-diffraction Far-field Imaging of Luminescent Single-Walled Carbon Nanotubes » L. Cognet, D. Tsyboulski, and R.B. Weisman Nanoletters, 8, 2 (2008) 749 - 753 |
