SINGLE-MOLECULE APPLICATIONS IN NEUROSCIENCES
Over the last years, with colleagues in neuroscience at IINS (D. Choquet, L. Groc et al.) we demonstrated that single-molecule detection techniques allow direct measurement of the movements of individual surface receptors for neurotransmitter at the surface of live neurons with nanometer resolutions. These movements have been recorded by tracking several types of individual nanometer-sized fluorescent nano-probes, such as a fluorophore or a semiconductor nanocrystal ( quantum dots ).
We performed several demonstrations to improve single molecule detection in live neuronal samples and applied such concepts for fundamental understandings of basic processes in neurosciences.
Current projects aim developing such strategies using novel types of nano-probes for studies in thick brain tissues, in close collaboration with L. Groc at IINS (Bordeaux)
Over the last years, with colleagues in neuroscience at IINS (D. Choquet, L. Groc et al.) we demonstrated that single-molecule detection techniques allow direct measurement of the movements of individual surface receptors for neurotransmitter at the surface of live neurons with nanometer resolutions. These movements have been recorded by tracking several types of individual nanometer-sized fluorescent nano-probes, such as a fluorophore or a semiconductor nanocrystal ( quantum dots ).
We performed several demonstrations to improve single molecule detection in live neuronal samples and applied such concepts for fundamental understandings of basic processes in neurosciences.
Current projects aim developing such strategies using novel types of nano-probes for studies in thick brain tissues, in close collaboration with L. Groc at IINS (Bordeaux)
Review articles:
« The 2015 Super-Resolution Microscopy Roadmap »
S. W. Hell, S.J. Sahl, M. Bates, , X. Zhuang, R. Heintzmann, M.J Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S.J. Davis, C. Eggeling, D. Klenerman, K.I. Willig, G. Vicidomini, M. Castello, A. Diaspro, T. Cordes
J. App. Phys. D 48 (2015) 443001
« Probing the Dynamics of Protein-Protein Interactions at Neuronal Contacts by Optical Imaging. »
O. Thoumine, H. Ewers, M. Heine, L. Groc, R. Frischknecht, G. Giannone, C. Poujol, P. Legros, B. Lounis, L. Cognet, D. Choquet.
Chem Rev., 108, 5 (2008) 1565-1587
« Multiple Routes for Glutamate Receptor Trafficking : Surface Diffusion and Membrane Traffic Cooperate to Bring Receptors to Synapses. »
L. Cognet, L. Groc, B. Lounis & D. Choquet
Science STKE, 327 (2006) pe13.
« The 2015 Super-Resolution Microscopy Roadmap »
S. W. Hell, S.J. Sahl, M. Bates, , X. Zhuang, R. Heintzmann, M.J Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S.J. Davis, C. Eggeling, D. Klenerman, K.I. Willig, G. Vicidomini, M. Castello, A. Diaspro, T. Cordes
J. App. Phys. D 48 (2015) 443001
« Probing the Dynamics of Protein-Protein Interactions at Neuronal Contacts by Optical Imaging. »
O. Thoumine, H. Ewers, M. Heine, L. Groc, R. Frischknecht, G. Giannone, C. Poujol, P. Legros, B. Lounis, L. Cognet, D. Choquet.
Chem Rev., 108, 5 (2008) 1565-1587
« Multiple Routes for Glutamate Receptor Trafficking : Surface Diffusion and Membrane Traffic Cooperate to Bring Receptors to Synapses. »
L. Cognet, L. Groc, B. Lounis & D. Choquet
Science STKE, 327 (2006) pe13.
Some achievements:
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« Single-nanotube tracking reveals the nanoscale organization of the extracellular space in the live brain » A. G. Godin, J. A. Varela, Z. Gao, N. Danné, J. P. Dupuis, B. Lounis, L. Groc and L. Cognet Nature. Nanotechnology (2017) |
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« Targeting neurotransmitter receptors with nanoparticles in vivo allows single molecule tracking in acute brain slices » J. A. Varela, J. P. Dupuis, L. Etchepare, A. Espana, L. Cognet and L. Groc Nat. Commun. 7 (2016) 10947 |
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« Dynamic super-resolution imaging of endogenous proteins on living cells at ultra-high density. » G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A.I. Sobolevsky, M.P. Rosconi, E. Gouaux, R. Tampé, D. Choquet and L. Cognet Biophys. J., 99 , 4 (2010) 1303-1310, Featured Article |
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« Surface mobility of postsynaptic AMPARs tunes synaptic transmission. » M. Heine, L. Groc, R. Frischknecht, JC. Béïque, B. Lounis, G. Rumbaugh, RL. Huganir, L. Cognet, D. Choquet Science, 320, 5873 (2008) 201-5 |
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« Surface Trafficking of Neurotransmitter Receptor : Comparison between Single Molecule/Quantum dot Strategies» L. Groc, M. Lafourcade, M.Heine, M. Renner, V. Racine, J.-B. Sibarita, B. Lounis, D. Choquet & L. Cognet J. Neuroscience, 27, 46 (2007) 12433 |
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« NMDA receptor surface mobility depends on NR2A-2B subunits. » L. Groc, M. Heine, S.L. Cousins, F. A. Stephenson, B. Lounis, L. Cognet, and D. Choquet Proc. Natl. Acad. Sci. USA, 103, 49 (2006) 18769-74 |
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« Direct imaging of lateral movements of AMPA receptors inside synapses » C. Tardin*, L. Cognet*, C. Bats, B. Lounis & D. Choquet (* : equal contribution) EMBO Journal 22 (2003) 4656-4665 |
