PHOTOTHERMAL MICROSCOPY
Photothermal Microscopy is currently the most sensitive approach for the detection and spectroscopy of individual non-fluorescent nanoscale-objects in complex environments. Because of its extreme sensitivity and the photo-stability of the delivered signals, photothermal microscopy is particularly attractive for biological applications both for label-free imaging or when using different types of nano-probes/labels (see also this page).
Photothermal Microscopy is currently the most sensitive approach for the detection and spectroscopy of individual non-fluorescent nanoscale-objects in complex environments. Because of its extreme sensitivity and the photo-stability of the delivered signals, photothermal microscopy is particularly attractive for biological applications both for label-free imaging or when using different types of nano-probes/labels (see also this page).
Review articles:
« Photothermal microscopy: optical detection of small absorbers in scattering environments »
P. Vermeulen, L. Cognet, B. Lounis
J. Microscopy 254, 3 (2014) 115
« Tracking receptors using Individual fluorescent and non fluorescent nanolabels »
L. Cognet, B. Lounis, D. Choquet
Cold Spring Harb Protoc. 2 (2014) 207-13
« Ultra-sensitive detection of individual gold nanoparticles : spectroscopy and applications to biology. »
L. Cognet , B. Lounis
Gold Bull., 41, (2008) 139-146
« Photothermal Methods for Single Nonluminescent Nano-Objects »
L. Cognet, S. Berciaud, D. Lasne & B. Lounis
Anal. Chem., 80, 7 (2008) 2288 – 2294,
« Photothermal microscopy: optical detection of small absorbers in scattering environments »
P. Vermeulen, L. Cognet, B. Lounis
J. Microscopy 254, 3 (2014) 115
« Tracking receptors using Individual fluorescent and non fluorescent nanolabels »
L. Cognet, B. Lounis, D. Choquet
Cold Spring Harb Protoc. 2 (2014) 207-13
« Ultra-sensitive detection of individual gold nanoparticles : spectroscopy and applications to biology. »
L. Cognet , B. Lounis
Gold Bull., 41, (2008) 139-146
« Photothermal Methods for Single Nonluminescent Nano-Objects »
L. Cognet, S. Berciaud, D. Lasne & B. Lounis
Anal. Chem., 80, 7 (2008) 2288 – 2294,
Some achievements:
« Direct visualization of carbon nanotube degradation in primary cells by photothermal imaging »
J. Russier, L. Oudjedi, M. Piponnier, C. Bussy, M. Prato, K. Kostarelos, B. Lounis, A. Bianco and L. Cognet
Nanoscale (2017) DOI: 10.1039/C6NR09795B
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« Small gold nanorods with tunable absorption for photothermal microscopy in cells » E. S. Shibu, N. Varkentina, L. Cognet and B. Lounis Adv. Sci. 4 (2017) 1600280 |
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« Optical detection of individual ultra-short carbon nanotubes enables their length characterization down to 10 nm » Z. Gao, L. Oudjedi, R. Faes, F. Moroté, C. Jaillet, P. Poulin, B. Lounis, and L. Cognet Sci. Rep. 5 (2015) 17093 |
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« A highly specific gold nanoprobe for live-cell single-molecule imaging .» C. Leduc, S. Si, , J. Gautier, M. Soto-Ribeiro, B. Wehrle-Haller, A. Gautreau, G. Giannone, L. Cognet, and B. Lounis Nano Lett. 13, 4, (2013) 1489-1494 |
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« Label-free optical imaging of mitochondria in live cells » D. Lasne, G.A. Blab, Francesca De Giorgi, François Ichas, B. Lounis & L. Cognet Optics Express , 15, 21 (2007) 14184-93 |
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Single Nanoparticle Photothermal Tracking (SNaPT) of 5 nm gold beads in live cells. » D. Lasne, G.A. Blab, S. Berciaud, M. Heine, L. Groc, D. Choquet, L. Cognet & B. Lounis Biophys. J, 91 (2006) 4598-4604 |
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« Observation of intrinsic size effects in the optical response of individual gold nanoparticles »
S. Berciaud, L. Cognet, P. Tamarat & B. Lounis Nano Letters 5 (2005) 515-518 « Photothermal Absorption Spectroscopy of Individual Semiconductor Nanocrystals » S. Berciaud, L. Cognet & B. Lounis Nano Letters 5 (2005) 2160-2163 |
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« Photothermal heterodyne imaging of individual nonfluorescent nanoclusters and nanocrystals » S. Berciaud, L. Cognet, G. Blab & B. Lounis Phys. Rev. Lett. 93 (2004) 257402 « Photothermal heterodyne imaging of individual metallic nanoparticles : Theory versus experiment. » S. Berciaud, D. Lasne, G.A. Blab, L. Cognet & B. Lounis Phys.Rev. B 73 (2006) 045424 |
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« Single metallic nanoparticle imaging for protein detection in cells » L. Cognet, C. Tardin, D. Boyer, D. Choquet, P. Tamarat and B. Lounis Proc. Natl. Acad. Sci. USA 100 (2003) 11350-11355 |
